Journal of Advanced Research in Science and Technology
Volume 7, Numéro 2, Pages 62-75

A Biotechnological Test The Production Of Citric Acid By Using Two Strains Of Aspergillus Niger Atcc-16404 And Anss-b5 In Solid-state Fermentation By Dates

Authors : Djafri Kaouthar . Khemissat Elhayfa . Bergouia Meriem .


The objective of this study is to optimize the production of citric acid by using two strains of Aspergillus niger ATCC 16404 and ANSS-B, this strains were cultivated in a medium based on scrap dates flour. In this sense, different culture conditions such as water content, initial pH and nitrogen and phosphorus contents were optimized. The results obtained show the optimum conditions for maximum production of citric acid are: A water content of 70.0%, an initial pH of 2.5, a nitrogen source is yeast extract at a content of 3.0 g / Kg and a content of Potassium phosphate 2.5 g / Kg. Maximum amounts and yields of citric acid of 173.80 - 201.60 g / Kg and 66.84 - 73.31%, were obtained with these optimum conditions. Thus, it can be concluded from the results of the present study that flours obtained from date scrap can serve as a potential substrate for the production of citric acid by A. Niger ATCC 16404 and ANSS-B5 by Solid State Fermentation (SSF).


Valuation ; Citric acid ; Aspergillus Niger ; Date scrap ; Solid State Fermentation