Recherche Agronomique
Volume 3, Numéro 4, Pages 1-8
1999-05-04
Effects Of The Herbicide Dichlobenil On Mitotic Instability And Haploidization Of Diploid Strains Of Aspergillus Nidulans
Authors : Chibani A. .
Abstract
The herbicide dichlobenil was compared with the standard haploidizing chemicals, benomyl and p-fluorophenylalanine, for its effects on mitotic stability and haploidization of diploid colonies of A. nidulans. Three different diploid strains were used so that the influence of a gene conferring resistance to dichlobenil (dicA) on mitotic stability could also be determined. Diploid colonies homozygous for the nor¬mal allele (dicAs // dicAs) and the mutant allele (dicAr // dicAr) or heterozygous at this locus (dicAr // dicAs) were transferred w dishes of CM and CM supplemented with dichlobenil, benomyl or pfa and incubated at 37°C. The number of sectors sco-red after 4 and 8 days incubation was used as a measure for mitotic instability. This investigation has shown that dichlobenil causes an increase in the somatic instability of A. nidulans diploids, and is as effective as the haploidizing chemicals benomyl and p-fluorophenylalanine (pfa) in generating haploid sectors.
Keywords
Dichlobenil, Aspergillus nidulans, Mitotic instability, Haploidization benomyl, p-fluorophenylalanine.
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