AGROBIOLOGIA
Volume 11, Numéro 1, Pages 2305-2315
2021-06-30
Phytochemical Study And Evaluation Of Antioxidant Activity Of Sophora Japonica L. (fabaceae) Of The Region Of Boumerdes (algeria).
Authors : Benhabyles Bouttaba Narimen . Bouchenak Ouahiba . Boumaza Sarah . Laoufi Razika . Yahiaoui Karima . Sadaoui Nesrine . Toubal Souheyla . Arab Karim .
Abstract
Description of the subject. Natural substances derived from plant biomass have multiple interests that are used in biotechnology as in the food, cosmetic and pharmaceutical industries. Among these compounds we find a large part of the secondary metabolites which have especially been illustrated in therapy. Traditional herbal remedies have long been used without knowing what their actions were due to. Objectives. Studies of secondary metabolites are the subject of much research based on in vitro cultures. This is particularly the case for the phenolic compounds which are the subject of our study, compounds widely used in therapy as vasculoprotectors, anti-inflammatories, enzyme inhibitors, antioxidants and anti-radicals. In this context, the present work concerns a phytochemical and antioxidant study from the plant Sophora japonica traditionally used to treat several diseases. Methods. We focused our work on the extraction of polyphenols by the maceration method, as well as their identification. Quantification by spectrophotometric methods allowed us to determine the contents of total phenols by the Folin-Ciocalteu reagent, flavonoids by Aluminum trichloride, tannins condensed by vanillin, anthocyanins by the differential PH method, and as carotenoids by the mixture of solvents (hexane / acetone / ethanol). Research of the effect of the phenolic extract on the antioxidant power was carried out by five methods : the method of reduction of the free radical DPPH, the method of reduction of ferrous iron (FRAP), the bleaching test of β- carotene / linoleic acid, the ABTS radical cation reduction test and total antioxidant capacity. Results. The extraction of polyphenols yielded a métanolique extract with a good yield of about 66.5%. The determination of total polyphenols, flavonoids, condensed tannins, anthocyanins, carotenoids and the methanolic extract revealed respective contents of 0,011mg/g, 15,65mg/g, 0.038 mg/g, 0,085mg/g, 1.333mg/g of dry dry plant material. For the DPPH test, the results reveal that the methanolic extract has an anti-radical activity greater than that of the standards (BHT and ascorbic acid) (P≤0.01). The iron reduction technique FRAP revealed a higher reducing capacity of the methanolic extract compared to quercetin and rutin (P≤0.01). The β-carotene bleaching test also showed that quercetin is the most active as inhibitors of the oxidation of linoleic acid compared to the methanolic extract, rutin and BHT. For the ABTS ° + test, the results reveal that the methanolic extract of sophora pods is more active as an inhibitor compared to BHT. The total antioxidant capacity test also showed that the methanolic extract expresses strong antioxidant power. Conclusion. The results of this work have enabled us to affirm that the extract of the plant studied has very good antioxidant properties which could allow us to recommend them in biotechnology.
Keywords
Oxidative stress, antioxidant, polyphenol, DPPH, FRAP, ABTS, β-carotene, CAT
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