Revue des Sciences Fondamentales Appliquées
Volume 9, Numéro 1, Pages 392-410

Antioxidant, Antibacterial And Cell Toxicity Effects Of Polyphenols From Ahmeur Bouamer Grape Seed Extracts

Authors : Ghouila Z. . Laurent S. . Boutry S. . Vander Elst L. . Nateche F. . Muller R. N. . Baaliouamer A. .


In this work and for the first time, significant concentrations of total polyphenols and flavonoids from Vitis vinifera L. grape seed extracts were obtained (256.15 ± 17.40 mg GAE/gdm and 14.08 ± 0.64 mg CE/gdm, respectively).The LC/MS analysis revealed richness in procyanidins. For antioxidant, antimicrobial and antifungal effects, the grape seed extract (GSE) responded positively. At 100 μg/mL, GSE induced a moderate toxicity of the order of 3.88% on 3T6 cells at the first 24 hours of treatment, whereas, its prolonged effect to 48 hours reduced this toxicity to less than 0.5%. As for the anti-proliferative effect on tumoral cell lines, a cell death of 18.39% to 23.79% and 10.30% to 20.37% was registered respectively for HeLa and BCPAP cells during 24 and 48 hours of treatment. Consequently, it is possible to consider using GSE at lower concentrations as an anti-proliferative agent without losing sight of its benefic effect on healthy cells.


grape seed extract, 3T6 cell, antioxidant, antibacterial, anti-proliferation.

Rich Extract On Total Polyphenols And Antioxidant Activity Obtained By Conventional And Non-conventional Methods From Ahmeur Bouamer Grape Seed

Ghouila Z. .  Laurent S. .  Henoumont C. .  Vander Elst L. .  Muller R. N. .  Baaliouamer A. . 
pages 692-711.

Assessment Of Antioxidant And Antibacterial Activity Of Phoenix Dactylifera L. Seed Extracts: Perspective For The Development Of New Foods

Ait Ouahioune Lidia .  Bara Fatma .  Bariz Karim .  Houali Karim .  Djenane Djamel . 
pages 298-308.

Effects Of Extraction Methods On Total Polyphenols, Free Radical Scavenging And Antibacterial Activity Of Crude Extracts Of Cleome Arabica L. Growing In Oued Souf Region

Chouikh Atef .  Rebiai Abdelkrim .  Aref Mahdia .  Heded Mounira .  Adjal El Hadda .  Alia Fatma . 
pages 14-17.