Revue des Sciences Fondamentales Appliquées
Volume 8, Numéro 3, Pages 692-711

Rich Extract On Total Polyphenols And Antioxidant Activity Obtained By Conventional And Non-conventional Methods From Ahmeur Bouamer Grape Seed

Authors : Ghouila Z. . Laurent S. . Henoumont C. . Vander Elst L. . Muller R. N. . Baaliouamer A. .

Abstract

Conventional and non-conventional extraction methods were applied in the first time for the determination of total polyphenols from Ahmeur Bouamer, an anthoctonous variety grape seeds. Independently of the time and temperature, a positive effect of particle size parameter on maceration has been noted and relativly high concentrations in polyphenols for 500 μm (67.78 ± 0.46 mg GAE/gdm), 500-355 μm (90.95 ± 0.24 mg GAE/gdm) and 355-180 µm (114.82 ± 1.24 mg GAE/gdm) have been obtained. The higher value of yield was reached by the microwave extraction (37.74±0.32%), followed by the maceration (14.16±0.15%) and the ultrasound (6.70±0.25%). The appropriate extraction method to obtain a relatively strong antioxidant activity from seeds was the microwave with inhibition of 69.67±1.76 % and 78.05±2.05% of DPPH and ABTS+ free radicals, respectively.

Keywords

Ahmeur Bouamer, extraction, grape seeds, total polyphenols, antioxidant activity.

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