Algerian Journal of Biosciences
Volume 1, Numéro 2, Pages 68-75

Phytochemical Study, Nutritive Value, Antioxidant And Anti-inflammatory Activities Of Phenolic Extracts From Desert Plant Calligonum Comosum L'hér.

Authors : صالحة عوادي . Chouikh Atef . Chemsa Ahmed Elkhalifa . Aounallah Chahira . Aounallah Imane . Alia Fatma .

Abstract

The aim of this study is to the estimation of the nutritive values, phytochemical study and the antioxidant and anti-inflammatory activities of ethyl acetate, tannins and crudes extracts from the aerial part of Calligonum comosum L’Hér. Which grow in South East of Algeria. According to the results obtained from the estimation of nutritive value, the plant is rich in proteins and lipids and very poor in carbohydrates. The results also showed a difference in the content of polyphenols and flavonoids. The crude extracts were high in polyphenols content in the ethanol extract 170.74±5.8 mg EAG/g Extract, and the best value of flavonoids in methanol extract 28.22±0.25 mg QE/g Extract. Chromatographic analysis by HPLC of methanolic extract has identified four phenolic compounds out of 38 peaks as: Gallic acid, Chlorogenic acid, Caffeic acid and Vanillic acid. The antioxidant activity was evaluated by three tests, the results of the DPPH• test, showed that methanol extract had the best scavenging than the other extracts (IC50: 2.09±0.02 μg/ml), But, in the hemolysis test, the values of the percentage of hemolysis were approximately the same at concentration 1 mg/ml between the extracts and standard used. In the reducing power test, its results showed that the tannins extract had the highest reducing power 79.17±5.22 μg/ml at Abs700nm=0.5. As for anti-inflammatory activity, the methanol and flavonoids phase ethyl acetate extracts showed the best inhibitory effect against protein denaturation compared to other extracts. The effects were estimated 3.14mg E Diclofenac/mg Extract.

Keywords

Calligonum comosum L'Hér.; Nutritive values; HPLC; Antioxidant activity; anti-inflammatory activity.

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