Algerian Journal of Natural Products
Volume 5, Numéro 2, Pages 483-491

Total Phenolic, Flavonoid Contents And In-vitro Anti-inflammation Evaluation Of Ethanol Extracts Of Hibiscus Sabdariffa Calyx, Malus Domestica And Their 1:1 Extracts Blend On Protein Denaturation

Authors : Shallangwa Gideon Adamu . Ekwumemgbo Patricia Adama . Osu Usman Isa . Bolarin Olubunmi Olukemi . Moyosore Abdullahi .


Hibiscus sabdarifa and Malus domestica are well known and widely used herbs, which contains several interesting bioactive constituents and possesses health promoting properties. The aim of this study is to determine the total phenolic and flavonoid contents, evaluate and compare the anti-inflammatory effects of ethanol extracts of the two extracts and a 1:1 blend of the extracts against the denaturation of proteins in vitro. The respective extracts were analyzed for their contents of polyphenols and flavonoids. The test extracts and reference drug (Ibuprofen) of varying concentrations were also incubated with egg albumin under controlled experimental conditions and subjected to determination of absorbance to assess the anti-inflammatory property. The results obtained exhibited a concentration-dependent inhibition of protein denaturation by both extracts, the 1:1 blend as well as the reference drug. The EC50 for extracts as well as those for the blend and the reference drug were determined by the dose-response curve using Graphpad Prism 5.0 software.


Hibiscus sabdariffa; Malus domestica; flavonoids; ibuprofen; anti-inflammation;protein denaturation

Antioxidant Activity, Phenolic And Flavonoid Content Of Wild Alhagi Maurorum Root Plant Extracts

Al-rimawi Fuad .  Nejada Mohammad A. .  Khalid Mahmoud F. .  Warad Ismail . 
pages 80-88.

Total Phenolic Contents, Radical Scavenging And Cyclic Voltammetry Of Three Native Date (phoenix Dactylifera L.) Varieties Grown In Ouargla

Zineb Ghiabaa Zineb .  Yousfi Mohamed .  Saidi Mokhtar .  Hadjadj Mohamed .  Dakmouche Messaouda . 
pages 70-79.

Evaluation Of Antioxidant Capacity, Phenol And Flavonoid Contents Of Opuntia Streptacantha And Opuntia ficus Indica Fruits Pulp

Mabrouki Lamia .  Zougari Bouthayna .  Bendhifi Monia .  Borgi Mohamed A. . 
pages 2-8.