Volume 10, Numéro 3, Pages 2036-2043

Effects Of Chlorpyrifos On Brain Oxidant/antioxidant Parameters In Pregnant/lactating Rats And Their Offspring

Authors : Abderrahim Khawla . Bouanane Samira . Baba Ahmed Fatima Zohra . Merzouk Hafida .


Description of the subject. Several studies have reported that low doses of Chlorpyrifos (CPF) target the developing brain during the period which cell division is occurring. It is well established that oxidative stress and excitatory synaptic transmission are closely related. Their disturbances are involved in most acute and chronic diseases of the central nervous system. Objective. In the present study, we evaluated the effects of gestational CPF exposure on the oxidative stress in the offspring. Methods. Oral administration of this pesticide at doses of 5.4mg/kg b.wt (1/25LD50) and 13.5 mg/kg b.wt (1/10LD50) was given 1day/2 to female rats during the entire gestation and lactation period and their pups. Several markers of oxidative stress were assessed by measuring the concentrations of brain malondialdehyde (MDA), carbonyl proteins and the activities of SOD and Catalase in pups at birth (day 0), weaning (day21) and 3 months of age (day90). Results. These offspring had significantly higher brain MDA and carbonyl protein at days 21 and 90 compared with control offspring. At day 90, higher brain CAT and SOD activities was observed in experimental offspring compared with control offspring Conclusion. CPF is not considered to be teratogenic at dose 1/10LD50 and 1/25 LD50. However, the oxidative stress occurred during intra-uterine life, persisted through adulthood in offspring of rats exposed by gavage of CPF


Chlorpyrifos ; brain ; oxidative parameters ; antioxidant enzymes