Journal Algérien des Régions Arides
Volume 13, Numéro 1, Pages 121-128

Determination Of Genetics Relationships By Moleculars Markers Of Ssr Type, For Some Varieties Of Apricot (prunus Armeniaca L.) In The Area Of The Hodna, (m’sila), Algeria

Authors : Bahlouli Faycal . Kellou K. . Zedam A. . Slamani R. . Benmehaia R. . Tellache S. . Bendif E. . Ykhle N. .

Abstract

For long time studies of the genetic diversity of the apricot in Algeria are mainly based on phenotypic criteria. Some of these criteria are limited to well-defined stages of development that are highly sensitive to environmental factors. Indeed, these criteria are unstable, so it became necessary to consider more reliable traits to better characterize real taxonomic diversity. Markers with high mutation rates, such as microsatellites (SSR) used in our study have the ability to be a high polymorphism between individuals, so they are the markers of choice for studies of genetic diversity. In this study eight apricot genotypes were analyzed with 11 microsatellite primer pairs. PCR amplification of microsatellite regions was obtained polymorphic, clear and easily interpretable profiles. These primers produced 100 bands were used to study the genetic diversity of eight genotypes. For the 100 bands obtained, 40% of these fragments were polymorphic and 60% were monomorphic. The size of the bands generated by the 11 primers is variable; it ranges from 60 bp to 360 bp. The analyses of different outcomes have to classify the eight varieties into three distinct groups. These results indicate a strong genetic similarity between varieties, despite their different geographic origins sometimes. These results confirm the usefulness of microsatellites markers for the study of genetic diversity in apricot.

Keywords

Apricot, molecular markers, PCR, microsatellite, genetic diversity