Journal of Molecular and Pharmaceutical Sciences
Volume 2, Numéro 1, Pages 9-21
2023-12-31
Ames Test And Sos Chromotest To Evaluate The Genotoxicity Effect Of Synthesized Series Of Antibacterial Sulfonamides
Authors : Becheker Imène . Marref Salah Eddine . Melakhessou Mohamed Akram . Berredjem Malika . Berredjem Hajira .
Abstract
Genotoxic property of four new antibacterial sulfonamides 1a-d has been evaluated in this study using two standards genotoxicity assays: the Salmonella typhimurium mutagenicity assay or Ames test based on the use of Salmonella strains TA100, TA98 and TA1535, treated with and without metabolic activation (S9 mix fraction) and the SOS ChromtestTM Kit assay using Escherichia coli PQ 37. From the results of the Ames test we note that only 1c (N-(phenyl) sulfamide) showed no genotoxic effect, contrary to 1a [(N-(4-methoxyphenyl) sulfamide], 1b [(N-(3-fluorophenyl) sulfamide] and 1d [(N-(phenylethyl) sulfamide] that have showed genotoxic effect with and without metabolic activation. Results of the SOS Chromotest confirmed these obtained with the Ames test. Sulfonamides 2d-f, expressed the genotoxic potential by stimulating the production of β-galactosidase. Knowing that the genotoxic effect of a molecule is strictly linked to its carcinogenic potential. So, from our results, we suggested that only compound 1c was non-genotoxic and safe to be tested, eventually, in vivo. We conclude that genotoxic effect depends essentially on the structure and composition of the molecule.
Keywords
Sulfonamides ; Genotoxicity ; Ames test ; SOS Choromotest
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