Applied Biology in Saharan Areas
Volume 3, Numéro 2, Pages 101-115
2021-03-30
Antifungal Activity Of Matricaria Pubescens .desf Et Juniperus Oxycedrus And Control Of Mycotoxin-producing Molds.
Authors : Makhloufi Ahmed . Benkhedda Khaled . Bey Said . Benlarbi Larbi .
Abstract
This work is part of the development of two medicinal plants (Matricaria pubescens (Desf) and Juniperus oxycedrus), growing spontaneously in the region of Bechar and Ain Sefra respectively, in order to know their phytochemical compositions and their properties. antifungal and anti-mycotoxin control in vitro. Phytochemical screening revealed the presence of saponosides, tannins, flavonoids, steroids, fatty acids, volatile oils and triterpenes in both plants. The moisture content of J. oxycedrus contains slightly higher than M. pubescens (Desf) with rates of the order of 32.66% and 30.80% respectively. The antifungal activity of the extracts (aqueous, ethanolic and methanolic) of the aerial parts of the two plants was tested on five fungal strains: Aspergillus niger; Aspergillus flavus; Fusarium oxysporum f.s albedinis (FOA1 and FOA2) and Penicellium purperogenum by the method of evaluation of radial growth on solid medium as well as the antifungal effect was obtained by the development of biomass in liquid medium. With the exception of the aqueous extract of J. oxycedrus where a reduction in radial growth of the FOA2 strain was recorded, the other tests involving the aqueous extracts showed increases as a function of the concentrations. Based on the measurements of diameters at the end of the cultures compared to the controls, very important antifungal indices were noted for the five strains according to the concentration of the extracts, with percent inhibition of 100% on FOA with the metholic and ethanolic extract of M. pubescens (Desf) at concentrations of 1.86 and 1.57 mg / ml respectively. With regard to the liquid media, almost complete inhibition was observed for all the strains tested with concentrations of the order of 1.57 and 2.7 mg / ml relative to the methanolic and ethanolic extracts added to the two plants. The most effective inhibitory potency is that of the methanolic, ethanolic extract of M. pubescens, and the aqueous extract of J.oxycedrus at concentrations of 0.046 and 0.059 mg / ml, respectively. Key words: Matricaria pubescens, Juniperus oxycedrus, antifungal activity, anti-mycotoxin, methanolic extract, ethanolic extract, aqueous extract.
Keywords
Key words: Matricaria pubescens, Juniperus oxycedrus, antifungal activity, anti-mycotoxin, methanolic extract, ethanolic extract, aqueous extract.
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