Algerian Journal of Arid Environment “AJAE”
Volume 6, Numéro 2, Pages 16-23
2016-12-10

Planomonospora Sp. Pm18: Isolation And Taxonomy Of New Actinobacterial Strain Isolated From Algerian Saharan Soil



Authors : Chaabane Chaouch Fawzia . Bouznada Khaoula . Bouras Noureddine . Tata Samira . Meklat Atika . Mokrane Salim . Lamari Lynda . Kemassi Abdellah . Mathieu Florence . Sabaou Nasserdine .

Abstract

An actinobacterial strain, designated PM18, was isolated from a Saharan soil sample by agar plate dilution method on chitin-vitamin agar supplemented with polymyxin after pretreatment of the soil sample at 120°C (dry heat) for 1 h. The taxonomic status of this strain was determined basis on morphological and physiological characteristics and phylogenetic analysis based on the 16S rRNA gene sequence. Strain PM18 developed an extensively branched and non-fragmented substrate mycelium. The isolate was characterized by the presence of cylindrical monosporous sporangia, which were produced only on the aerial mycelium. Sporangia were arranged closely in parallel double rows. The monosporous sporangia were motile. The morphological features of this isolate corresponded to those of members of the genus Planomonospora. Furthermore, phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PM18 is a member of the genus Planomonospora. The 16S rRNA gene sequence similarity showed that this strain was most closely related to Planomonospora sphaerica JCM 9374T (99.2%) and Planomonospora parontospora subsp. antibiotica JCM 3094T (98.8%). Based on phenotypic differences and the separate position of strain PM18 in the phylogenetic tree, this strain may be a representative of a putative novel species in the genus Planomonospora

Keywords

Planomonospora, Actinobacteria, taxonomy, Saharan soil, Microbial diversity

Les articles similaires

Utilisation Of Agro Alimentary Wastes For Bio Surfactants ‎production By A Thermophilic Bacterial Strain Novelty ‎isolated From An Algerian Crude Oil Contaminated Soil

Mokdad ‎ K .  Mesbaiah F.z. ‎ .  Eddouaouda K .  Badis A . 
pages 40-45.

Diversity Of Actinobacteria In Algerian Saharan Soil And Description Of Sixteen New Taxa

Bouras Noureddine .  Meklat Atika .  Boubetra Dalila .  Saker Rafika .  Boudjelal Farida .  Aouiche Adel .  Lamari Lynda .  Zitouni Abdelghani .  Schumann Peter .  Spröer Cathrin .  Klenk Hans-peter .  Sabaou Nasserdine . 
pages 61-78.

The Effect Of Thermic Pretreatment And Antibiotics On The Selective Isolation Of The Culturable Actinomycetes From The Algerian Desert Soil

Boudemagh A .  Bensouici K . 
pages 25-32.

Malachite Green Decolorization By Marine Strain Isolated From Bou-ismail Bay (algeria)

Mesbaiah Fatma Zohra .  Badis A. . 
pages 58-62.

Isolation, Identification And Study Of The Probiotic Criteria Of Lactobacillus Plantarum Isolated From The Raw Goat Milk Of The Five Farms Of The Oran Region

Mami A. . 
pages 25-32.

Formats de citation