Synthèse
Volume 10, Numéro 1, Pages 7-9
2004-06-30

Biological Activity Of Some Moulting Hormone Agonists In Mealworms: Ecdysteroid And Protein Analysis In Ovaries



Authors : Soltani-mazouni N. . Boukachabia A. . Smagghe G. .

Abstract

In a topical (I 0 ug/Icmale) bioassay, three moulting hormone agonisrs (RH-0345, RH-5849. RII-5992) were tested on newly emerged adult females of the mealworrn, Tenebrio molitor (Coleoptera: Tcnebrionidae), under in vivo conditions. In a first series of experiments the compounds were assayed on ovarian ecdystcroid production measured at day 2 following treatment, corresponding to the beginning of vitellogenesis. Data from enzyme immunoassay measurements revealed that all tested compounds resulted in a significant increase in ovarian ecdysteroid amounts. Moreover, RH-5992 was found more active than RH-0345 and RH-5849. In a second series of experiments. the activity of these compounds was investigated on ovarian proteins. The ecdystoroid agonist RII-5849 caused a significant reduction of protein amounts in the ovaries. The electrophoretic separation •of ovarian extracts from treated series showed that one or two protein bands were missing compared to controls. These observations may suggested an interference of ecdysteroid agonists with the vitellogenesis process

Keywords

Mealworm, Insecticides. Ovaries, Hormones. Ecdysteroids, Proteins

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