Sciences & technologie. C, Biotechnologies
Volume 0, Numéro 42, Pages 38-43
2015-12-31

Importance Of The Bioinformatics Tool In The Confirmation Of Bacterial Species Isolated From Raw Milk : Case Of Stenotrophomonas Spp.

Authors : Boubendir Abdelhafid . Khelili Kaoutar . Hamidechi Mohamed Abdelhafid .

Abstract

The present study concerned the biomolecular and bioinformatic characterization of psychrotrophic bacteria resistant to cephazolin, in particular Stenotrophomonas spp.The raw milk samples (N = 64) of 30 cows were collected in Biskra, in the South-East of Algeria. All the characteristics tested allowed the presumptive identification of Stenotrophomonas spp. The sequence of the 16S rRNA was used on the BlastN bioinformatics tool of the NCBI portal. The results of the alignment with GenBank 16S RNA sequences confirmed the species: it is Stenotrophomonasmaltophilia.

Keywords

Stenotrophomonasmaltophilia, BlastN, 16S RNA