Revue des Substances Naturelles et Innovation Thérapeutique
Volume 2, Numéro 1, Pages 13-21
2016-01-30

Antimicrobial Activity Of Polyphenol Extracts From Algerian Ficus Carica Dried Fruit.



Authors : Debib A. . Tir-touil A. . Meddah B. .

Abstract

In vitro antimicrobial and chemical properties of methanol, acetone, Petroleum ether and aqueous extract of dried figs against 9 strains resistant human pathogens were evaluated. An Algerian variety fig was extracted with water, methanol, Petroleum ether and 80% acetone. Preliminary phytochemical analysis of extracts revealed the presence of antimicrobial compounds such as tannins, flavonoids and coumarins, The total amount of polyphenols (milligrams of gallic acid equivalents (GAE) per 100 g dried fruit) in fig fruit extracts was determined by using the Folin-Ciocalteu assay, Tannins were determined by precipitation with casein or hide powder and flavonoids by the aluminium trichloride method. The antimicrobial activity of the extracts was evaluated and based respectively on the inhibition zone using the disc-diffusion assay, minimal inhibition concentration (MIC), minimum bactericidal concentration (MBC) assay. Results showed that the phenolic compounds and flavonoids were abundant in acetone and Petroleum ether extract when compared to other extracts. The four extracts possessed antibacterial activity against tested Grampositive and Gram-negative bacteria particularly Petroleum ether their broad spectrums may be due to the presence of coumarins and flavonoids. Candida albicans was more susceptible to all figs extracts.

Keywords

Ficus carica (dried figs), Antimicrobial activity, MIC/MBC, “Taamriout” Algerian variety.

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