PhytoChem & BioSub Journal
Volume 8, Numéro 3, Pages 190-197
2014-11-25
Determination Of Caffeic Acid And Gallic Acid In Algerian Bee Pollen By An Hplc Method
Authors : Rebiai Abdelkerim . Lanez Touhami . Belfar Mohamed Lakhder .
Abstract
Bee pollen is a fine powder-like material produced by flowering plants pollen, mixed with nectar and bee secretions and gathered by the honey-bees. Pollens are the male reproductive cells of flowers and bees' primary food source, containing concentrations of phytochemicals and nutrients and rich in secondary metabolites. It is known that bee pollen contains lipids, sugars, proteins, amino acids, flavonoid and phenolic acids such as caffeic acid and gallic acid. The aim of our study is to evaluate caffeic and gallic acids from different sorts of bee pollen from Algeria using an HPLC method. Analysis of caffeic acid, and gallic acid was realized with the chromatographic column VPODS RP18 type and using a gradient of mobile phase (Acetonitril- 0,1% acetic acid) and spectrophotometric detection. Caffeic acid in the range from 24.734 to 42.182 μg/g and gallic acid from 0.321 to 0.406 mg/g was determined in tested pollen samples.
Keywords
: Bee pollen, HPLC, phenolic acids, caffeic acid, gallic acid.
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