Revue Nature et Technologie
Volume 6, Numéro 1, Pages 29-33
2014-06-30
Auteurs : Bengueddour Yassir. . El Han Safâa . El Ibaoui Hamid. . El Ayadi Rachida. . Brhadda Najiba. .
This work lies within the scope of the valorization of the extracts of sesquipedalian Rhodophycées Gelidium as antioxydants. The method applied to measure this activity is that of the trapping of the free radicals by using the 1,1'-diphenyl-2 - picrylhydrazyl (DPPH°) and the follow-up of the reaction was carried out by colorimetry and Liquid Chromatography High efficiency. The results obtained show that the extracts of Gelidium sesquipedale have a significant antioxidant activity, inhibition of 50% of oxidation of the radical has been achieved by using a dilution of 100 times of the extracts. The characterisation of molecules responsible for this biological activity has shown that extracts of Gelidium sesquipedale are rich in phenolic compounds, ascorbic acid and functions thiols. The levels recorded for these molecules are of the order of 8.71 ± 6.1 mg/g of EAG after crushing for the total polyphenols, 5.35 ± 4.77 mg/ml for the ascorbic acid, and 8.65 ± 1.12 mg/ml of equivalent of N- acetylcysteine conjugate for the functions thiols.
Sesquipedalian Gelidium; DPPH method total Polyphenols, Ascorbic acid, Functions thiols, HPLC, Colorimetry.
Benchabane Othmane
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Hazzit Mohameda
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Bousta Lilianea
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Abbou Brahim
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pages 23-28.
Benchabane Othmane
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Hazzit Mohamed
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Bousta Liliane
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Abbou Brahim
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pages 23-28.
Baguia-broune Fatou Diane Micheline
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N’gaman Kouassi Kohué Christelle Chantal
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Virieux David
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Békro Yves-alain
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pages 25-30.
Didi M.a
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Makhoukhi B
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Villemin D
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pages 9-14.
Belkhouche N.
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Didi M. A.
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pages 7-14.