Revue des Sciences Fondamentales Appliquées
Volume 9, Numéro 2, Pages 908-924
2017-05-01
Authors : Abdullah F. I. . Chua L. S. . Rahmat Z. .
This study investigated several extraction methods for proteins from the leaves of Ficus deltoidea. The protocols include solvent based extraction, TCA-acetone precipitation, Tris buffered phenol extraction and hybrid technique of TCA-acetone/phenol-SDS. The results indicated that the hybrid technique and Tris buffered phenol method could produce higher number and better quality of proteins. There are 22 protein bands with the wide range of molecular size ranging from 8.20 to 113.48 kDa separated by 12% polyacrylamide gel electrophoresis in the hybrid technique. Tris buffered phenol could extract 13 protein bands from the plant, but only 9 protein bands from TCA-acetone precipitation method. pH 8.0 was the optimum value of Tris buffered phenol for protein extraction with higher protein content and better gel resolution.
Ficus deltoidea; protein extraction; TCA-acetone; phenol-SDS; pH.
بوسالم أحلام
.
عابد يوسف
.
ص 117-132.
Yahia Zeghoudi
.
pages 74-88.
Said Houari Amel
.
pages 257-268.
Chebouti Yahia
.
Mihoubi Aissa
.
Maamar-kouadri Kaddour
.
Ifticene-habani Naima
.
Chebouti-meziou Nadjiba
.
pages 109-119.
Louaer Mehdi
.
Zermane Ahmed
.
Larkech Ouassila
.
Besombes Colette
.
Allaf Karim
.
Meniai Abdeslam Hassan
.
pages 32-35.