Algerian Journal of Health Sciences
Volume 3, Numéro 4, Pages 41-50
2021-12-31

Association Study Of Angiotensin-converting Enzyme Insertion/deletion Gene Polymorphism With Neovascular Age-related Macular Degeneration In A Sample Of Algerian Population

Authors : Abid Ghania . Messal Ahlem . Harmel Mohammed . Missouri Mohammed . Djadaouadji Iness . Adda Neggaz Nawel . Idder Aicha . Abdi Meriem . Meroufel Djabaria Naima . Fodil Mostafa . Zemani-fodil Faouzia .

Abstract

Introduction: Neovascular age-related macular degeneration (nAMD) is a progressive ocular disease and the major cause of central visual loss and blindness. Genetic factors play an important role in pathogenesis process of this disease. Findings on the presence of renin-angiotensin system (RAS) components including angiotensin-converting enzyme gene in the ocular tissues and its inflammatory response, suggest that deregulation of RAS may enhance the risk of AMD. The aim of our study is to investigate the association of ACE I/D (rs1799752) polymorphism with Neovascular Age-related Macular Degeneration (nAMD) in a sample of Algerian patients. Material and Methods: This prospective study consisted of 72 patients with nAMD and 72 control subjects. DNA of 144 subjects was extracted using Salting Out method. Genotyping of I/D polymorphism of the ACE gene was carried out using multiplex polymerase chain reaction method. Statistical analysis was performed by SPSS.21.0 to evaluate the association of ACE I/D polymorphism with the risk of developing nAMD. Results: There was no difference of ACE I/D (rs1799752) genotypic (p=0.1, OR=0.5 [0.2-1.2]) and allelic (p=0.8, OR=0.8 [0.01-1.1]) distributions between patient and control groups. Stratification by age and by gender did not show any significant association between ACE I/D (rs1799752) polymorphism and nAMD. Conclusion: ACE I/D (rs1799752) polymorphism was not associated with nAMD in our sample of Algerian population. It would be interesting to study the impact of other genes involved in the renin angiotensin system.

Keywords

Neovascular age-related macular degeneration ; ACE gene ; polymorphism ; multiplex PCR ; Algerian population

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