AGROBIOLOGIA
Volume 11, Numéro 2, Pages 2715-2724

Gc-ms Profiling Of Transformed Roots Of Calotropis Procera

Authors : Djerdjouri Amina . Abbad Mohamed . Boumrah Yacine .

Abstract

Description of the subject: Calotropis procera (Ushaar) is a xerophyte species containing secondary metabolites that confer a number of biological and pharmacological properties to be used as a potential source of disease-treatment drugs. A number of studies have reported on the chemical composition of Ushaar leaves but there are no reports on hairy roots composition. Objective: The aim of this study was thus to analyze the phytochemical composition of the hairy roots of C. procera induced by genetic transformation via Agrobacterium rhizogenes. Methods: Induced hairy roots (4 weeks old) have been dried, grounded into fine powder and solubilized into a solvent solution containing 20% dichloromethane, 50% acetonitrile, 10% ethanol, and 20% hexane. The extracts have been analyzed by Gas chromatography (GC) and mass spectrometry (MS). Results: Different chemical compounds that belong to different classes of secondary metabolites have been identified. Those identified compounds include fatty acids, sterols, tri-terpenes, alkanes and esters. The most abundant components were; palmitic acid (23.15%); linoleic acid isomers 1 and 2 (14.36%, 12.18% respectively); squalene (8.60%); stigmasterol (12.96%); β-sitosterol (5.07%) and campesterol (4.93%). Conclusion: The identified compounds have various therapeutic effects as anticancer, anti-inflammatory and antibacterial molecules. The ability to induce hairy roots containing active compounds suggests the possibility of using Calotropis as a source of potential therapeutic metabolites through a plant molecular pharming setting.

Keywords

Calotropis procera ; Apocynaceae ; hairy roots ; GC-MS ; secondary metabolites ; ushaar