Volume 11, Numéro 2, Pages 2628-2640

Production Of Biosurfacant From A Novel Bacillus Amylolequifaciens Strain K2e:potential Application As Thickener Of Saled Dressings

Auteurs : Khelouia Lamia . Eddouaouda Kamel . Mechri Sondes . Hamiche Sonia . Zenati Billal . Mesbaiah Fatima Zohra . Jaouadi Bassem . El Hattab Mohamed . Badis Abdelmalek .


In the current study, in total, 77 hydrocarbon-degrading bacteria previously isolated from contaminated soil in the Algerian desert (Hassi-Messaoud, petroleum region), were individually screened for biosurfactant production. An isolate (designated as K2E) showed a particular performance in production of potent biosurfactant, able to reduce surface tension (ST) from 72 mN/m to 30.32 mN/m. The phylogenetic analysis showed that strain K2E was very closely related to Bacillus amylolequifaciens (identity of 98.28%). This biosurfactant was produced by culturing K2E strain in mineral salt medium (MSM) using olive oil (1%) as the only source of carbon and energy, yielded a maximum biosurfactant concentration (0.8 g/L) and having a critical micelle concentration (CMC) (0.75 g/L).The produced biosurfactant showed a good stability at extreme environmental conditions [temperatures (from 0 °C to 120 °C), pH (2–12) and salinity (up to 200 g/L)], over a time period up to 3 years. The product obtained by dichloromethane extractor, is linked in lipopetide biosurfactant group as indicated by Fourier Transform Infrared (FTIR) spectroscopy.

Mots clés

Biosurfactant ; Production ; Bacillus amylolequifaciens ; Thickener ; Food industry