Revue Nature et Technologie
Volume 13, Numéro 2, Pages 61-73

Preliminary Screening And Characterization Of Novel Proteolytic Enzymes Produced By Extremophilic Bacteria Isolated From Tunisian And Algerian Biotopes

Authors : Mechri Sondes . Bouacem Khelifa . Allala Fawzi . Khaled Marwa . Bouanane-darenfed Amel . Hocine Hacene . Jaouadi Bassem .


The current paper reports on the production and characterization of extracellular thermostable proteases produced from thermophilic bacteria namely Aeribacillus pallidus VP3, Anoxybacillus kamchatkensis M1V, Virgibacillus natechei FarDT, and Melghiribacillus thermohalophilus Nari2AT strains isolated from different extremophlic biotopes in Tunisia and Algeria. The key challenge of the use of the different proteases in industrial applications is their efficiency under unconventional conditions. An optimization via one-factor-at-a-time (OFAT) methodology has been used to obtain 3,000 U.mL-1; 4,600 U.mL-1; 15,800 U.mL-1; and 16,000 U.mL-1 of proteases activity from VP3, M1V, FarDT, and Nari2AT strains, respectively. Particularly, VP3 and FarDT proteases showed a high tolerance to several organic solvents. In addition, the protease produced from FarDT strain might be useful as potential candidate for application in detergency. The deproteinization of shrimp wastes by M1V and Nari2AT proteases was successful with a high rate of deproteinization of about 48% and 82%. The obtained proteolytic hydrolysate obtained by M1V strain showed high biological activities with half maximal inhibitory concentration (IC50) of 71.52 μg.mL-1, 107.67 μg.mL-1, and 133.24 μg.mL-1, respectively for the angiotensin, tyrosinase, and amylase inhibitory activities.


Extremophilic bacteria ; thermostable proteases ; biotechnologically applications

Comparison Of Maldi Tof Ms Profiling And 16s Rrna Gene Identification Of Presumptive Lactic Acid Bacteria Isolated From The Traditional Algerian Date Product “btana”.

Abekhti Abdelkader .  Cnockaert Margo .  Aerts Maarten .  Daube Georges .  Vandamme Peter .  Tahri Assia .  Taminiau Bernard . 
pages 27-43.

Phenotypic Identification And Whole Cell Protein Analysis By Sds-page Of Dominants Lactic Acid Bacteria Isolated From Algerian Raw Milk

Ghazi Fatima .  Benmechernene Zineb .  Aggad Habib .  Guessas Bettache .  Moussa-boudjamaa Boumedien .  Henni Djamal Eddine .  Kihal Mebrouk . 
pages 11-22.

Identification Of Bacterial Strains Isolated From The Traditional Date Product “btana” Produced In South Regions Of Algeria.

Abekhti Abdelkader .  Taminau Bernard .  Djebbar Abla .  Crevecoeur Sebestian .  Daube Georges . 
pages 11-26.