Applied Biology in Saharan Areas
Volume 3, Numéro 3, Pages 11-26

Identification Of Bacterial Strains Isolated From The Traditional Date Product “btana” Produced In South Regions Of Algeria.

Authors : Abekhti Abdelkader . Taminau Bernard . Djebbar Abla . Crevecoeur Sebestian . Daube Georges .

Abstract

Eleven samples of the traditional date product “ Btana” prepared by direct (DBM) and undirect method (UBM) were analysed to characterize their bacterial diversity. The total aerobic count was ranged from 1,6 . 102 to 3,02 . 106 UFC/g. Two hundred isolates (200) were randomly selected, then 37 representative strains have been chosen for molecular identification. 16S rRNA gene sequencing revealed the presence of 17 species within 30.9% belonged to the main Bacillus species; B. pumilus, B. endophytucis and B amyloliquefaciens. The other identified species included Staphylococcus (S. epidermidis S. pasteuri S capitis ), Enterococcus (E. faecalis E. mundtii, E. faecium. Within the minority represented species, we recorded the presence of Paenibacillus (P. xylanexedens; P. macerans), Streptococcus (S. salivarius; S. lactis), Lactobacillus sakei and Klebsiella pneumoniae. Our results indicated that UBM is more selective for spore former bacilli contrary to DBM that shows more diversity in the bacterial flora with Enterococcus being the prevailing species. Enzymatic profil revealed a weak hydrolytic activity for the dominant Bacillus which exhibit instead a high lypolitic activity and other enzymes including proteases.

Keywords

Btana ; Date fruit ; traditional food ; , bacterial diversity ; , food safety