Applied Biology in Saharan Areas
Volume 3, Numéro 3, Pages 1-10

Isolation Of Acanthamoeba Castteloni In Sudanese Corneal Ulcers Patients -omdurman –sudan.

Authors : Yousif Lowaty Lowba Jamal . Hamid Sulimanabdallah Hamid . Abdel Salam Basheir Sati Abdel Salam .


Abstract. Background. Acanthamoeba Keratitis is infection of theeye corneaof human by the free-living protozoan amoeba belonged to the genus Acanthamoeba ubiquitous in variety of environments and can lead to sever disease including granulomatous encephalitis. Objective:this study aimed to isolate Acanthamoebakeratits in scraped samples from patients suffering corneal ulcer in Sudan. Material and methods: This cross sectional study conducted in Omdurman eye hospitals (Makka and Elwalidain) in aperiod of time from 2017 to 2020. A total of 90 corneal scrapes samples collected and examined by direct microscopy and cultivation in 1.5% non-nutrient agar seeded withEscherichia coli heat –killed bacteria. Results: A total of 90 corneal scrapes samples obtainedfrom patient’s sufferers from corneal ulcer. Thetrophozoite and cyst of acanthamoeba were seen in direct microscopy examination in14 samples out of 90.Out of 14 positive samples, 11(78.6%) were males and 3 (21.4%) were femalesThe 14 positive samples by direct microscopy were cultured in non-nutrient agar media,10 (71.43%) were positive culture, 4 (23.57%) negative culture Conclusion: Corneal ulcers. Requiresearly recognition and prompt management to minimize the impactof the disease .Accidental corneal trauma by exposed to foreignbody and contact lens wear is primary risk factors, early prognosis is always utmost priority to treatment and culture techniqueis more sensitive, reliable andefficacy for highly significant in detection Acanthamoeba keratitis. Keywords: Acanthamoeba keratitis,isolation, riskfactor. Corneal ulcer.


Acanthamoeba keratitis,isolation, riskfactor. Corneal ulcer.