Applied Biology in Saharan Areas
Volume 2, Numéro 1, Pages 17-25

Physicochemical Characterization And Antimicrobial Activity Of Essential Oil From Rosmarinus Officinalis L, Growing In The Wild

Authors : Makhloufi Ahmed . Boulanouar Ali .


The antimicrobial activity of Rosmarinus officinalis L essential oil has been recognized for a long time. In the present study, the antimicrobial properties of the essential oils obtained from the leaves of R. officinalis L, have been investigated against six strains of bacteria and six fungi strains. R. officinalis L, provided a yield of 1.5% of essential oil. The Physicochemical characterization shows that refractive index is about (1.467). Specific density at 20 °C (0.949), optical rotation (+11.7), colour and odour were also determined. R. Officinalis L. essential oil exhibited stronger antimicrobial activity, with a maximum inhibition zone of 28 mm against E. Faecalis. In addition, the essential oil has a remarkable antifungal activity. P. Escpansum is the most sensitive strain with an inhibition diameter of 24 mm. All fungal strains were inhibited at a low concentration of 1/600.


Essential oil, Rosmarinus officinalis L, antimicrobial activity, physicochemical characterization.