Applied Biology in Saharan Areas
Volume 1, Numéro 2, Pages 23-29
2017-12-15
The Effect Of Cotula Cinerea Essential Oil On Spore Germination,mycelial Growth And On Sporulation Pf Phytopathogenic Fungi
Authors : Benahmed Fatiha .
Abstract
Abstract – This work aimed to evaluate the antifungal activity of Cotula cinerea essential oil against Aspergillus niger. Essential oil from the aerial parts of the plant was obtained by hydrodistillation and the antifungal activity was investigated by agar dilution method on spore germination, mycelial growth and on sporulation of A. niger using different concentrations (1/100, 1/250, 1/500, 1/1000, 1/2000, 1/5000 and 1/10000 (v/v)). Results showed that the essential oil yield was 1,42% (v/w). The results from this study demonstrated that oil extract was effective in inhibiting spore germination, radial growth and sporulation of A. niger. The inhibitory effect of extract increase when concentration increased. Complete inhibition of conidial germination and mycelial growth was found at 1/250 and 1/100 respectively. But in case of sporulation, 80% was the most inhibition rate. This study suggests that the essential oil of aerial parts of C. cinerea have antifungal activity and could be an al-ternative treatment in controling A. niger.
Keywords
Keywords: Antifungal properties, Aspergillus niger, Cotula cinerea, Essential oil.
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