algerian journal of environmental science and technology
Volume 5, Numéro 2, Pages 933-955
2019-06-30
Characterization By Spectrometric Methods Of Chitin Produced From White Shrimp Shells Of Parapenaeus Longirostris Bylactobacillus Helveticus Cultivated On Glucose Or Date Waste
Authors : . Arbia W . Arbiab L . Adour L . Amrane A. . Benhadji A. . Lounic H .
Abstract
Chitin recovery by lactic acid fermentation was considered. It was previously shown that the use of glucose led to 61% of demineralization and 42% of deproteinization; while and unlike glucose, the inoculation of L. helveticus in date waste (200 g L-1 reducing sugars) led to a simultaneous demineralization and deproteinization of shrimp shells, 83 and 61 %, respectively. The purity of end-products were characterized by infrared (FTIR), X-ray diffraction and X-ray fluorescence. Analysis of the chemical composition of fermentation end-product by X-ray fluorescence showed that residual minerals are in trace except CaO and P2O5. The infrared spectra and the X-ray diffractograms of both samples compared to those of pure chitin showed a strong similarity. FTIR of fermented shells showed the appearance of characteristic peaks of α-chitin and the disappearance of calcite peaks. In addition and especially for the shells fermented using L. helveticus cultivated on date waste, a similarity with those of pure chitin should be noted. These results confirmed the efficiency of biological chitin recovery, and that a synthetic carbon source (glucose) can be replaced by a natural one, date waste, allowing a valorization of this latter. The relevance of subsequent works to improve yields of demineralization and deproteinization was therefore confirmed.
Keywords
Biopolymer recovery; Structural characterization; Bioprocess; Agricultural waste
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